Its new site will open on Regent Street on 3 June serving its range of healthy salads and hot dishes.
It will be followed by sites in Monument, Moorgate, Covent Garden and Battersea that are due to open by the end of the year.
The opening menu of the Regent Street site will feature an exclusive bowl featuring blackened chicken with romaine, baby spinach, cherry tomatoes, avocado, parmesan, crispy shallots and buffalo Caesar dressing alongside favourites such as the Azetca and Seiz’a Caesar salads.
"We are very excited to open atis on Regent Street: it marks a very exciting milestone in our journey," says co-founder Eleanor Warder.
"Our new flagship store embodies our commitment to offering fresh, vibrant and delicious food whilst creating an energetic and welcoming environment for our community."
Atis was founded in Shoreditch in October 2019 and now operates sites in Belgravia, Notting Hill, Canary Wharf and Borough Yards.