The brand - which is known for its botanically-themed interiors and cocktail offer - currently operates 23 sites with further locations set to launch in Cardiff, Ipswich and Chester soon.
Natasha Waterfield, chief operating officer at New World Trading Company said: “Bringing our unique concepts to new cities and towns for the first time is always an exciting moment for the business.”
“The vision for The Glass Works aligns with our brand and was an opportunity not to be missed following the impressive regeneration in the town. We can’t wait for the residents of Barnsley to experience our one-of-a-kind offer at The Botanist.”
Cllr Sir Stephen Houghton, leader of Barnsley Council, said: “The Botanist is undeniably one of the most impressive bar-and-restaurant chains in the country and we are thrilled to add them to the mix at The Glass Works.”
“When we set about regenerating the town centre several years ago, it was our ambition to create an environment that helped us attract brands of standout quality. To see this vision become a reality, with The Botanist complementing the likes of Cineworld, Superbowl UK, Nando’s, Next and River Island, is extremely rewarding."